New Publication

An Aptamer-Based gFET-Sensor for Specific Quantification of Gene Therapeutic Human Adenovirus Type 5, Li et al. Biosensors 2025, 15 (9), 605.

The integration of reduced graphene oxide field-effect transistors (rGO-FETs) with DNA-oligonucleotide aptamers offers a powerful method for analyte detection, providing both high sensitivity and selectivity. Using human adenovirus type 5 (HAdV-5) as a model target, our new study by Runliu Li, Ann-Kathrin Kissmann, Hu Xing, Roger Hasler, Christoph Kleber, Wolfgang Knoll, Hannes Schmietendorf, Tatjana Engler, Lea Krutzke, Stefan Kochanek and Frank Rosenau demonstrates how aptamer/FET systems can detect this clinically and biotechnologically important viral vector. A specialized anti-HAdV-5 aptamer library was generated through nine rounds of SELEX, enabling specific fluorescent labeling of HAdV-5 and related subtypes. This library also proved effective as the recognition element on a gFET sensor, allowing sensitive quantification of viral particles. Since adenoviruses are widely employed as vectors in gene therapy and genetic vaccines - including those used during the COVID-19 vaccination campaigns - there is a growing demand for reliable monitoring in production processes. gFET-based aptasensors show strong potential as tools for real-time monitoring in viral manufacturing workflows.

Link to publication: https://www.mdpi.com/2079-6374/15/9/605